Optimisation and automation of microscopy workflows for near-real-time data assessment and visualisation.

An informational Q&A session for those interested in learning how to optimise microscopy workflows in their institution. This event was run by Joshua Silver (University of Wollongong) in June 2023.

Watch the recording of this session to learn more about Optimisation and automation of microscopy workflows.

To access presentation slides click here.

Over the last few years, technological progress and optimisation of workflows at the Cryogenic Electron Microscopy at the University of Wollongong have enabled instrument scientists and researchers to assess the quality of cryoEM datasets in minutes rather than days. Much of this near-real-time data revolution has been made possible by automating and optimising a range of tasks involved in the early steps of data capture from microscopes.

In this presentation, Joshua Silver explained how existing workflows were reassessed in order to enable the near-real-time assessment and visualisation of cryoEM data. In particular, he highlighted the challenges that needed to be overcome. Finally, Josh demonstrated how the lessons learnt in optimising and automating cryoEM workflows can be applied to other instruments and workflows from data capture through to transfer, processing and visualisation.

This work was undertaken as part of the Australian Characterisation Commons at Scale (ACCS) project, under Work Package 4: “Big-data electron and correlative microscopy from instrument to publication”.

About the speaker:

Joshua Silver is a Research Data Officer at the University of Wollongong. He has 25 years experience with Unix System Administration, Network design and macOS Support. He is currently assisting researchers at the University of Wollongong with managing their data storage and workflow needs.